α-Amylase Inhibition, Antioxidant Activity and Phytochemical Analysis of Calotropis gigantea (L.) Dryand

Authors

  • Rajendra Gyawali Kathmandu University
  • Bijay Bhattarai Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Susan Bajracharya Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Surakshya Bhandari Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Puja Bhetwal Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Kanchan Bogati Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Shardul Neupane Department of Biotechnology, Kathmandu University, Dhulikhel, Nepal
  • Sahana Shrestha Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Ashwinee Kumar Shrestha Department of Pharmacy, Kathmandu University, Dhulikhel, Nepal
  • Rajendra Joshi Department of Chemical Science and Engineering, Kathmandu University, Dhulikhel, Nepal
  • Prem Narayan Paudel Department of Chemical Science and Engineering, Kathmandu University, Dhulikhel, Nepal

DOI:

https://doi.org/10.37107/jhas.143

Keywords:

Calotropis gigentea, alpha-Amylase inhibitory, Antioxidant activity, Fractionation, GC-MS, 5-Hydroxyl methyl furfural

Abstract

Introduction: Antioxidant and α-Amylase inhibitory activity of methanolic extract of Calotropis gigantea (L.) Dryand leaves were evaluated.

Methods: The antioxidant activity was evaluated by DPPH assay. The extract was fractionated in Silica gel loaded column chromatography (CC). All fractions were evaluated for their purity by TLC. Out of 11 fractions from CC, one fraction was analyzed by Gas Chromatography-Mass Spectrometry (GC-MS).

Results: The antioxidant activity of methanolic extract was found satisfactory (IC50268.80 µg/ml) as compared with ascorbic acid (141.82 µg/ml). TLC of a fractions showed a compound at Rf value at 0.45 in toluene: chloroform: methanol with mobile phase ratio 7:2:1 respectively.

Conclusions: Total 17 compounds were identified by GC-MS of ethyl acetate fraction and 5-hydroxyl methyl furfural was major furan compound (59.49%). α-Amylase inhibitory activity of the same fraction showed IC50 value of 0.94 mg/ ml. The Nepalese originated C. gigentea (L.) Dryand possesses antioxidant and α-Amylase inhibitory property.

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Published

2020-06-02

How to Cite

Gyawali, R., Bhattarai, B. ., Bajracharya, S. ., Bhandari, S. ., Bhetwal, P. ., Bogati, K. ., Neupane, S. ., Shrestha, S. ., Shrestha, A. K. ., Joshi, R. ., & Paudel, P. N. . (2020). α-Amylase Inhibition, Antioxidant Activity and Phytochemical Analysis of Calotropis gigantea (L.) Dryand . Journal of Health and Allied Sciences, 10(1), 77–81. https://doi.org/10.37107/jhas.143

Issue

Vol. 10 No. 1 (2020)

Section

ORIGINAL ARTICLES